FITC-inulin is synthesized by reacting fluoresceinyl isothiocyanate (FITC) with inulin (from dahlia tubers). The product is a yellow powder which is readily soluble in water.
The molecular weight as determined by Size Exclusion Chromatography calibrated with dextran standards is approximately 4000. Since dextran and inulin have different hydrodynamic volumes, this value is not absolute.
Inulin has long been used for monitoring renal clearance. FITC-inulin offers a simple and sensitive alternative to the cumbersome chemical assays of inulin and also permits real time analyses. The fluorescence properties are similar to FITC-dextran.
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For notes on the crystallinity of inulin see data file below.