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FITC-Polysucrose

Polysucrose (renamed from Ficoll™) is a polymer synthesized by cross-linking sucrose with epichlorohydrin. Polysucrose is readily soluble in water and salt solutions over a wide range of pH. As with sucrose, Polysucrose is more sensitive to acid than dextran so that care must be taken when working at acid pH. 

Polysucrose 70 is a fraction of Polysucrose with a molecular weight of 70 000. The Stokes radius is ca. 50Å which is lower that that (58Å) for dextran 70 and is attributed to the fact that Polysucrose has a more spherical structure and is less flexible. Polysucrose displays excellent biocompatablity. FITC-Polysucrose 70 is prepared by reacting Polysucrose 70 with FITC under similar conditions to those used for FITC-dextrans. It is supplied as a yellow powder which is freely soluble in water. 

FITC-Polysucrose fractions with weight average molecular weight 20 000, 40 000 and 400 000 are also available. 

If you need larger quantities please contact us for a bulk quotation.

Ficoll™ is a trademark owned by GE Healthcare.

References

Have you cited this product in a publication? Let us know so we can reference it.

  1. Monfrini, M. et al. Therapeutic potential of Mesenchymal Stem Cells for the treatment of diabetic peripheral neuropathy. Experimental Neurology 288, 75–84 (2017).
  2. Dolinina, J., Sverrisson, K., Rippe, A., Öberg, C. M. & Rippe, B. Nitric oxide synthase inhibition causes acute increases in glomerular permeability in vivo, dependent upon reactive oxygen species. American Journal of Physiology-Renal Physiology 311, F984–F990 (2016).
  3. Sverrisson, K., Axelsson, J., Rippe, A., Asgeirsson, D. & Rippe, B. Acute reactive oxygen species (ROS)-dependent effects of IL-1β, TNF-α, and IL-6 on the glomerular filtration barrier (GFB) in vivo. American Journal of Physiology-Renal Physiology 309, F800–F806 (2015).
  4. Nääv, Å. et al. A1M Ameliorates Preeclampsia-Like Symptoms in Placenta and Kidney Induced by Cell-Free Fetal Hemoglobin in Rabbit. PLOS ONE 10, e0125499 (2015).
  5. Axelsson, J., Rippe, A. & Rippe, B. mTOR inhibition with temsirolimus causes acute increases in glomerular permeability, but inhibits the dynamic permeability actions of puromycin aminonucleoside. American Journal of Physiology-Renal Physiology 308, F1056–F1064 (2015).
  6. Wester-Rosenlöf, L. et al. A1M/α1-Microglobulin Protects from Heme-Induced Placental and Renal Damage in a Pregnant Sheep Model of Preeclampsia. PLOS ONE 9, e86353 (2014).
  7. Sverrisson, K., Axelsson, J., Rippe, A., Asgeirsson, D. & Rippe, B. Dynamic, size-selective effects of protamine sulfate and hyaluronidase on the rat glomerular filtration barrier in vivo. American Journal of Physiology-Renal Physiology 307, F1136–F1143 (2014).
  8. Axelsson, J., Rippe, A., Sverrisson, K. & Rippe, B. Scavengers of reactive oxygen species, paracalcitol, RhoA, and Rac-1 inhibitors and tacrolimus inhibit angiotensin II-induced actions on glomerular permeability. American Journal of Physiology-Renal Physiology 305, F237–F243 (2013).
  9. Srikantha, N. et al. Influence of molecular shape, conformability, net surface charge, and tissue interaction on transscleral macromolecular diffusion. Experimental Eye Research 102, 85–92 (2012).
  10. Hutchens, M. P., Fujiyoshi, T., Komers, R., Herson, P. S. & Anderson, S. Estrogen protects renal endothelial barrier function from ischemia-reperfusion in vitro and in vivo. American Journal of Physiology-Renal Physiology 303, F377–F385 (2012).
  11. Axelsson, J., Öberg, C. M., Rippe, A., Krause, B. & Rippe, B. Size-selectivity of a synthetic high-flux and a high cut-off dialyzing membrane compared to that of the rat glomerular filtration barrier. Journal of Membrane Science 413–414, 29–37 (2012).
  12. Axelsson, J., Rippe, A., Öberg, C. M. & Rippe, B. Rapid, dynamic changes in glomerular permeability to macromolecules during systemic angiotensin II (ANG II) infusion in rats. American Journal of Physiology-Renal Physiology 303, F790–F799 (2012).
  13. Sangalli, F. et al. Effect of ACE inhibition on glomerular permselectivity and tubular albumin concentration in the renal ablation model. American Journal of Physiology-Renal Physiology 300, F1291–F1300 (2011).
  14. Axelsson, J., Sverrisson, K., Rippe, A., Fissell, W. & Rippe, B. Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo. American Journal of Physiology-Renal Physiology 301, F708–F712 (2011).
  15. Axelsson, J., Rippe, A. & Rippe, B. Transient and sustained increases in glomerular permeability following ANP infusion in rats. American Journal of Physiology-Renal Physiology 300, F24–F30 (2010).
  16. Axelsson, J., Rippe, A. & Rippe, B. Acute hyperglycemia induces rapid, reversible increases in glomerular permeability in nondiabetic rats. American Journal of Physiology-Renal Physiology 298, F1306–F1312 (2010).
  17. Jeansson, M., Björck, K., Tenstad, O. & Haraldsson, B. Adriamycin Alters Glomerular Endothelium to Induce Proteinuria. JASN 20, 114–122 (2009).
  18. Grände, G. et al. Unaltered size selectivity of the glomerular filtration barrier in caveolin-1 knockout mice. American Journal of Physiology-Renal Physiology 297, F257–F262 (2009).
  19. Goldberg, S., Harvey, S. J., Cunningham, J., Tryggvason, K. & Miner, J. H. Glomerular filtration is normal in the absence of both agrin and perlecan–heparan sulfate from the glomerular basement membrane. Nephrol Dial Transplant 24, 2044–2051 (2009).
  20. Axelsson, J., Rippe, A., Venturoli, D., Swärd, P. & Rippe, B. Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats. American Journal of Physiology-Renal Physiology 296, F242–F248 (2009).
  21. Axelsson, J., Mahmutovic, I., Rippe, A. & Rippe, B. Loss of size selectivity of the glomerular filtration barrier in rats following laparotomy and muscle trauma. American Journal of Physiology-Renal Physiology 297, F577–F582 (2009).
  22. Asgeirsson, D., Axelsson, J., Rippe, C. & Rippe, B. Similarity of permeabilities for Ficoll, pullulan, charge-modified albumin and native albumin across the rat peritoneal membrane. Acta Physiologica 196, 427–433 (2009).
  23. Rippe, C., Rippe, A., Torffvit, O. & Rippe, B. Size and charge selectivity of the glomerular filter in early experimental diabetes in rats. American Journal of Physiology-Renal Physiology 293, F1533–F1538 (2007).
  24. Corna, D. et al. Effects of Rosuvastatin on Glomerular Capillary Size-Selectivity Function in Rats with Renal Mass Ablation. AJN 27, 630–638 (2007).
  25. Asgeirsson, D., Venturoli, D., Fries, E., Rippe, B. & Rippe, C. Glomerular sieving of three neutral polysaccharides, polyethylene oxide and bikunin in rat. Effects of molecular size and conformation. Acta Physiologica 191, 237–246 (2007).
  26. Rippe, C., Rippe, A., Larsson, A., Asgeirsson, D. & Rippe, B. Nature of glomerular capillary permeability changes following acute renal ischemia-reperfusion injury in rats. American Journal of Physiology-Renal Physiology 291, F1362–F1368 (2006).
  27. Rippe, C., Asgeirsson, D., Venturoli, D., Rippe, A. & Rippe, B. Effects of glomerular filtration rate on Ficoll sieving coefficients (θ) in rats. Kidney International 69, 1326–1332 (2006).
  28. Jeansson, M., Granqvist, A. B., Nyström, J. S. & Haraldsson, B. Functional and molecular alterations of the glomerular barrier in long-term diabetes in mice. Diabetologia 49, 2200–2209 (2006).
  29. Figliuzzi, M. et al. Biocompatibility and function of microencapsulated pancreatic islets. Acta Biomaterialia 2, 221–227 (2006).
  30. Asgeirsson, D., Venturoli, D., Rippe, B. & Rippe, C. Increased glomerular permeability to negatively charged Ficoll relative to neutral Ficoll in rats. American Journal of Physiology-Renal Physiology 291, F1083–F1089 (2006).
  31. Guimarães, M. A. M., Nikolovski, J., Pratt, L. M., Greive, K. & Comper, W. D. Anomalous fractional clearance of negatively charged Ficoll relative to uncharged Ficoll. American Journal of Physiology-Renal Physiology 285, F1118–F1124 (2003).

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FITC-Polysucrose 40 (FP40)

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FITC-Polysucrose 170 (FP170)

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FITC-Polysucrose 400 (FP400)

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