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FITC-Inulin

FITC-inulin is synthesized by reacting fluoresceinyl isothiocyanate (FITC) with inulin (from dahlia tubers). The product is a yellow powder which is readily soluble in water.

The molecular weight as determined by Size Exclusion Chromatography calibrated with dextran standards is approximately 4000. Since dextran and inulin have different hydrodynamic volumes, this value is not absolute. 

Inulin has long been used for monitoring renal clearance. FITC-inulin offers a simple and sensitive alternative to the cumbersome chemical assays of inulin and also permits real time analyses. The fluorescence properties are similar to FITC-dextran. 

If you need larger quantities please contact us for a bulk quotation.

For notes on the crystallinity of inulin see data file below.

References

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  1. Pennings, I. et al. Layer-specific cell differentiation in bi-layered vascular grafts under flow perfusion. Biofabrication (2019). doi:10.1088/1758-5090/ab47f0
  2. Palygin, O. et al. Essential role of Kir5.1 channels in renal salt handling and blood pressure control. JCI Insight 2 (2019)
  3. Ilatovskaya, D. V. et al. Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC). EBioMedicine 40, 663–674 (2019).
  4. Spires, D. et al. Protective role of Trpc6 knockout in the progression of diabetic kidney disease. American Journal of Physiology-Renal Physiology 315, F1091–F1097 (2018).
  5. Andreev-Andrievskiy, A. A., Popova, A. S., Lagereva, E. A. & Vinogradova, O. L. Fluid shift versus body size: changes of hematological parameters and body fluid volume in hindlimb-unloaded mice, rats and rabbits. Journal of Experimental Biology 221, jeb182832 (2018).
  6. Mollet, B. B., Bogaerts, I. L. J., Almen, G. C. van & Dankers, P. Y. W. A bioartificial environment for kidney epithelial cells based on a supramolecular polymer basement membrane mimic and an organotypical culture system. Journal of Tissue Engineering and Regenerative Medicine 11, 1820–1834 (2017).
  7. Dolinina, J., Sverrisson, K., Rippe, A., Öberg, C. M. & Rippe, B. Nitric oxide synthase inhibition causes acute increases in glomerular permeability in vivo, dependent upon reactive oxygen species. American Journal of Physiology-Renal Physiology 311, F984–F990 (2016).
  8. Sverrisson, K., Axelsson, J., Rippe, A., Asgeirsson, D. & Rippe, B. Acute reactive oxygen species (ROS)-dependent effects of IL-1β, TNF-α, and IL-6 on the glomerular filtration barrier (GFB) in vivo. American Journal of Physiology-Renal Physiology 309, F800–F806 (2015).
  9. Nääv, Å. et al. A1M Ameliorates Preeclampsia-Like Symptoms in Placenta and Kidney Induced by Cell-Free Fetal Hemoglobin in Rabbit. PLOS ONE 10, e0125499 (2015).
  10. Nakano, D. et al. Reduction of Tubular Flow Rate as a Mechanism of Oliguria in the Early Phase of Endotoxemia Revealed by Intravital Imaging. JASN 26, 3035–3044 (2015).
  11. Wester-Rosenlöf, L. et al. A1M/α1-Microglobulin Protects from Heme-Induced Placental and Renal Damage in a Pregnant Sheep Model of Preeclampsia. PLOS ONE 9, e86353 (2014).
  12. Sverrisson, K., Axelsson, J., Rippe, A., Asgeirsson, D. & Rippe, B. Dynamic, size-selective effects of protamine sulfate and hyaluronidase on the rat glomerular filtration barrier in vivo. American Journal of Physiology-Renal Physiology 307, F1136–F1143 (2014).
  13. Sverrisson, K. et al. Extracellular fetal hemoglobin induces increases in glomerular permeability: inhibition with α1-microglobulin and tempol. American Journal of Physiology-Renal Physiology 306, F442–F448 (2013).
  14. Axelsson, J., Rippe, A., Sverrisson, K. & Rippe, B. Scavengers of reactive oxygen species, paracalcitol, RhoA, and Rac-1 inhibitors and tacrolimus inhibit angiotensin II-induced actions on glomerular permeability. American Journal of Physiology-Renal Physiology 305, F237–F243 (2013).
  15. Sandoval, R. M. et al. Multiple Factors Influence Glomerular Albumin Permeability in Rats. JASN 23, 447–457 (2012).
  16. Axelsson, J., Öberg, C. M., Rippe, A., Krause, B. & Rippe, B. Size-selectivity of a synthetic high-flux and a high cut-off dialyzing membrane compared to that of the rat glomerular filtration barrier. Journal of Membrane Science 413–414, 29–37 (2012).
  17. Axelsson, J., Rippe, A., Öberg, C. M. & Rippe, B. Rapid, dynamic changes in glomerular permeability to macromolecules during systemic angiotensin II (ANG II) infusion in rats. American Journal of Physiology-Renal Physiology 303, F790–F799 (2012).
  18. Axelsson, J., Sverrisson, K., Rippe, A., Fissell, W. & Rippe, B. Reduced diffusion of charge-modified, conformationally intact anionic Ficoll relative to neutral Ficoll across the rat glomerular filtration barrier in vivo. American Journal of Physiology-Renal Physiology 301, F708–F712 (2011).
  19. Axelsson, J., Rippe, A. & Rippe, B. Transient and sustained increases in glomerular permeability following ANP infusion in rats. American Journal of Physiology-Renal Physiology 300, F24–F30 (2010).
  20. Axelsson, J., Rippe, A. & Rippe, B. Acute hyperglycemia induces rapid, reversible increases in glomerular permeability in nondiabetic rats. American Journal of Physiology-Renal Physiology 298, F1306–F1312 (2010).
  21. Grände, G. et al. Unaltered size selectivity of the glomerular filtration barrier in caveolin-1 knockout mice. American Journal of Physiology-Renal Physiology 297, F257–F262 (2009).
  22. Axelsson, J., Mahmutovic, I., Rippe, A. & Rippe, B. Loss of size selectivity of the glomerular filtration barrier in rats following laparotomy and muscle trauma. American Journal of Physiology-Renal Physiology 297, F577–F582 (2009).
  23. Rippe, C., Rippe, A., Torffvit, O. & Rippe, B. Size and charge selectivity of the glomerular filter in early experimental diabetes in rats. American Journal of Physiology-Renal Physiology 293, F1533–F1538 (2007).
  24. Rippe, C., Rippe, A., Larsson, A., Asgeirsson, D. & Rippe, B. Nature of glomerular capillary permeability changes following acute renal ischemia-reperfusion injury in rats. American Journal of Physiology-Renal Physiology 291, F1362–F1368 (2006).
  25. Rippe, C., Asgeirsson, D., Venturoli, D., Rippe, A. & Rippe, B. Effects of glomerular filtration rate on Ficoll sieving coefficients (θ) in rats. Kidney International 69, 1326–1332 (2006).

 

 

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FITC-Inulin (FI)

FITC-Inulin (FI)

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