NEWS: TdB Consultancy are happy to announce that we now are producing TRITC-dextran following an improved procedure yielding even brighter fluorescent products. The improved degree of substitution of pure Tetramethylrhodamine dye on high quality dextran will enhance reproducibility and accuracy of in vitro and in vivo experiments.
Tetramethyl-rhodamine B is conjugated to dextran fractions and Polysucrose fractions by means of a stable thiocarbamate linkage. A mixture of 5- and 6-amino-tetramethyl-rhodamine B is converted to the isothiocyanate (TRITC) which is then allowed to reacted with dextran giving TRITC-dextran as a red powder.
The excitation maximum for TRITC is 550 nm and the emission maximum is 572 nm. Interestingly, the fluorescence is less dependent on pH than for fluorescein. A slight increase is noted around pH 6-7.
We are now presenting fixable fluorescent dextran derivatives - please see below "TRITC Lysine Dextran". Cell and tissue fixation is performed in order to preserve components in a “life-like state” and to make cells permeable to allow antibodies to access cellular structures, for example for microscopy studies or immunostaining